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GL Biochem p-map4 (s760) antibody
A , B Representative bands and corresponding quantification of p-MAP4, p-MAP4 (S737), and <t>p-MAP4(S760)</t> in the skin tissues of MAP4 KI mice. *** p < 0.001 versus the WT group. n = 5. C Representative bands and corresponding statistical analysis of LC3 in skin tissues. ** p < 0.01 versus the WT group. n = 5. D , E Representative images and corresponding quantifications of autophagosomes. Yellow arrows pointed to autophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. F Representative bands and corresponding statistical analysis of LC3 in primary MKs. ** p < 0.01 versus the WT group. n = 5. G , H Representative bands and corresponding quantifications of autophagosomes in primary MKs. Yellow arrows pointed to autophagosomes/mitophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. I , J Representative images and corresponding quantifications of GFP-LC3 positive keratinocytes. *** p < 0.001 versus WT group. n = 5. K , L Representative images and corresponding quantifications of autophagosome or autolysosome in keratinocytes measured by mRFP-GFP-LC3. Scale bar = 10 μm. * p < 0.05 and *** p < 0.001 versus WT group. n = 5. M Representative images of co-staining p-MAP4 with mitophagosomes. Scale bar = 10 μm. n = 5. N , O Representative bands and corresponding quantifications of p-MAP4 in keratinocytes treated by hypoxia with or without 30 min pre-treatment of 40 μM MDIVI-1. * p < 0.05 and *** p < 0.001 versus Con group. ### p < 0.001 versus Hypoxia (24 h) group. n = 5.
P Map4 (S760) Antibody, supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p-map4 (s760) antibody/product/GL Biochem
Average 90 stars, based on 1 article reviews
p-map4 (s760) antibody - by Bioz Stars, 2026-06
90/100 stars

Images

1) Product Images from "Mitophagy associated self-degradation of phosphorylated MAP4 guarantees the migration and proliferation responses of keratinocytes to hypoxia"

Article Title: Mitophagy associated self-degradation of phosphorylated MAP4 guarantees the migration and proliferation responses of keratinocytes to hypoxia

Journal: Cell Death Discovery

doi: 10.1038/s41420-023-01465-3

A , B Representative bands and corresponding quantification of p-MAP4, p-MAP4 (S737), and p-MAP4(S760) in the skin tissues of MAP4 KI mice. *** p < 0.001 versus the WT group. n = 5. C Representative bands and corresponding statistical analysis of LC3 in skin tissues. ** p < 0.01 versus the WT group. n = 5. D , E Representative images and corresponding quantifications of autophagosomes. Yellow arrows pointed to autophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. F Representative bands and corresponding statistical analysis of LC3 in primary MKs. ** p < 0.01 versus the WT group. n = 5. G , H Representative bands and corresponding quantifications of autophagosomes in primary MKs. Yellow arrows pointed to autophagosomes/mitophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. I , J Representative images and corresponding quantifications of GFP-LC3 positive keratinocytes. *** p < 0.001 versus WT group. n = 5. K , L Representative images and corresponding quantifications of autophagosome or autolysosome in keratinocytes measured by mRFP-GFP-LC3. Scale bar = 10 μm. * p < 0.05 and *** p < 0.001 versus WT group. n = 5. M Representative images of co-staining p-MAP4 with mitophagosomes. Scale bar = 10 μm. n = 5. N , O Representative bands and corresponding quantifications of p-MAP4 in keratinocytes treated by hypoxia with or without 30 min pre-treatment of 40 μM MDIVI-1. * p < 0.05 and *** p < 0.001 versus Con group. ### p < 0.001 versus Hypoxia (24 h) group. n = 5.
Figure Legend Snippet: A , B Representative bands and corresponding quantification of p-MAP4, p-MAP4 (S737), and p-MAP4(S760) in the skin tissues of MAP4 KI mice. *** p < 0.001 versus the WT group. n = 5. C Representative bands and corresponding statistical analysis of LC3 in skin tissues. ** p < 0.01 versus the WT group. n = 5. D , E Representative images and corresponding quantifications of autophagosomes. Yellow arrows pointed to autophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. F Representative bands and corresponding statistical analysis of LC3 in primary MKs. ** p < 0.01 versus the WT group. n = 5. G , H Representative bands and corresponding quantifications of autophagosomes in primary MKs. Yellow arrows pointed to autophagosomes/mitophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. I , J Representative images and corresponding quantifications of GFP-LC3 positive keratinocytes. *** p < 0.001 versus WT group. n = 5. K , L Representative images and corresponding quantifications of autophagosome or autolysosome in keratinocytes measured by mRFP-GFP-LC3. Scale bar = 10 μm. * p < 0.05 and *** p < 0.001 versus WT group. n = 5. M Representative images of co-staining p-MAP4 with mitophagosomes. Scale bar = 10 μm. n = 5. N , O Representative bands and corresponding quantifications of p-MAP4 in keratinocytes treated by hypoxia with or without 30 min pre-treatment of 40 μM MDIVI-1. * p < 0.05 and *** p < 0.001 versus Con group. ### p < 0.001 versus Hypoxia (24 h) group. n = 5.

Techniques Used: Staining



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GL Biochem p-map4 (s760) antibody
A , B Representative bands and corresponding quantification of p-MAP4, p-MAP4 (S737), and <t>p-MAP4(S760)</t> in the skin tissues of MAP4 KI mice. *** p < 0.001 versus the WT group. n = 5. C Representative bands and corresponding statistical analysis of LC3 in skin tissues. ** p < 0.01 versus the WT group. n = 5. D , E Representative images and corresponding quantifications of autophagosomes. Yellow arrows pointed to autophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. F Representative bands and corresponding statistical analysis of LC3 in primary MKs. ** p < 0.01 versus the WT group. n = 5. G , H Representative bands and corresponding quantifications of autophagosomes in primary MKs. Yellow arrows pointed to autophagosomes/mitophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. I , J Representative images and corresponding quantifications of GFP-LC3 positive keratinocytes. *** p < 0.001 versus WT group. n = 5. K , L Representative images and corresponding quantifications of autophagosome or autolysosome in keratinocytes measured by mRFP-GFP-LC3. Scale bar = 10 μm. * p < 0.05 and *** p < 0.001 versus WT group. n = 5. M Representative images of co-staining p-MAP4 with mitophagosomes. Scale bar = 10 μm. n = 5. N , O Representative bands and corresponding quantifications of p-MAP4 in keratinocytes treated by hypoxia with or without 30 min pre-treatment of 40 μM MDIVI-1. * p < 0.05 and *** p < 0.001 versus Con group. ### p < 0.001 versus Hypoxia (24 h) group. n = 5.
P Map4 (S760) Antibody, supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p-map4 (s760) antibody/product/GL Biochem
Average 90 stars, based on 1 article reviews
p-map4 (s760) antibody - by Bioz Stars, 2026-06
90/100 stars
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A , B Representative bands and corresponding quantification of p-MAP4, p-MAP4 (S737), and p-MAP4(S760) in the skin tissues of MAP4 KI mice. *** p < 0.001 versus the WT group. n = 5. C Representative bands and corresponding statistical analysis of LC3 in skin tissues. ** p < 0.01 versus the WT group. n = 5. D , E Representative images and corresponding quantifications of autophagosomes. Yellow arrows pointed to autophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. F Representative bands and corresponding statistical analysis of LC3 in primary MKs. ** p < 0.01 versus the WT group. n = 5. G , H Representative bands and corresponding quantifications of autophagosomes in primary MKs. Yellow arrows pointed to autophagosomes/mitophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. I , J Representative images and corresponding quantifications of GFP-LC3 positive keratinocytes. *** p < 0.001 versus WT group. n = 5. K , L Representative images and corresponding quantifications of autophagosome or autolysosome in keratinocytes measured by mRFP-GFP-LC3. Scale bar = 10 μm. * p < 0.05 and *** p < 0.001 versus WT group. n = 5. M Representative images of co-staining p-MAP4 with mitophagosomes. Scale bar = 10 μm. n = 5. N , O Representative bands and corresponding quantifications of p-MAP4 in keratinocytes treated by hypoxia with or without 30 min pre-treatment of 40 μM MDIVI-1. * p < 0.05 and *** p < 0.001 versus Con group. ### p < 0.001 versus Hypoxia (24 h) group. n = 5.

Journal: Cell Death Discovery

Article Title: Mitophagy associated self-degradation of phosphorylated MAP4 guarantees the migration and proliferation responses of keratinocytes to hypoxia

doi: 10.1038/s41420-023-01465-3

Figure Lengend Snippet: A , B Representative bands and corresponding quantification of p-MAP4, p-MAP4 (S737), and p-MAP4(S760) in the skin tissues of MAP4 KI mice. *** p < 0.001 versus the WT group. n = 5. C Representative bands and corresponding statistical analysis of LC3 in skin tissues. ** p < 0.01 versus the WT group. n = 5. D , E Representative images and corresponding quantifications of autophagosomes. Yellow arrows pointed to autophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. F Representative bands and corresponding statistical analysis of LC3 in primary MKs. ** p < 0.01 versus the WT group. n = 5. G , H Representative bands and corresponding quantifications of autophagosomes in primary MKs. Yellow arrows pointed to autophagosomes/mitophagosomes. Scale bar = 0.5 μm. *** p < 0.001 versus WT group. n = 5. I , J Representative images and corresponding quantifications of GFP-LC3 positive keratinocytes. *** p < 0.001 versus WT group. n = 5. K , L Representative images and corresponding quantifications of autophagosome or autolysosome in keratinocytes measured by mRFP-GFP-LC3. Scale bar = 10 μm. * p < 0.05 and *** p < 0.001 versus WT group. n = 5. M Representative images of co-staining p-MAP4 with mitophagosomes. Scale bar = 10 μm. n = 5. N , O Representative bands and corresponding quantifications of p-MAP4 in keratinocytes treated by hypoxia with or without 30 min pre-treatment of 40 μM MDIVI-1. * p < 0.05 and *** p < 0.001 versus Con group. ### p < 0.001 versus Hypoxia (24 h) group. n = 5.

Article Snippet: MAP4 (1:1000, Affinity, USA), p-MAP4 (1:1000, GL Biochem, CHN), p-MAP4 (S760) (1:1000, GL Biochem, CHN), p-MAP4 (S737) (1:1000, GL Biochem, CHN), LC3 (1:1000, sigma, USA), p62 (1:1000, sigma, USA), Beclin1 (1:1000, CST, USA), Bcl-2 (1:1000, CST, USA), VDAC1 (1:1000, Millipore, USA), Tomm20 (1:1000, Affinity, USA), TIM23 (1:1000, Affinity, USA), GM130 (1:1000, Affinity, USA), Calnexin (1:1000, Affinity, USA), HA-tag (1:1000, proteintech, USA) and β-Actin (1:1000, proteintech, USA).

Techniques: Staining